Edaravone Continuous Infusion Toxicology in Rats

• Gynecologic Oncology
• Published: 31 March 2022

Protective effect of edaravone on cisplatin-induced injury in rat ovary

Archives of Gynecology and Obstetrics volume 306,pages 1673–1678 (2022)Cite this article

• 184 Accesses

• 1 Citations

• Metrics details

Abstract

Purpose

This study was aimed to evaluate the protective effect of edaravone on cisplatin-induced ovarian injury.

Methods

A total 40 female Wistar–Albino rats were utilized to form four groups: Group 1 (control group) (n = 10), no procedure was performed. Group 2 (cisplatin group) (n = 10), single-dose 7.5 mg/kg cisplatin was administered and no procedure was performed. Group 3 (edaravone group) (n = 10), single-dose 1 mg/kg edaravone was administered and no procedure was performed. Group 4 (cisplatin + edaravone group) (n = 10), single-dose 7.5 mg/kg cisplatin and 1 mg/kg edaravone were administered. Seventy-two hours later, ovaries were surgically extirpated in all groups. Malondialdehyde (MDA) levels and nitric oxide (NO) levels were studied in blood samples. In ovarian tissue samples, DNA damage and apoptosis were assessed using TUNEL method. Ovarian tissue damage was evaluated by immunohistochemical staining with caspase 3 and caspase 8.

Results

According to the findings obtained from the study, edaravone showed protective properties on ovarian damage due to cisplatin. MDA and NO levels were significantly higher in cisplatin group than other groups. Histopathological ovarian tissue damage in the cisplatin group was significantly higher than other groups. Similarly, DNA damage and apoptosis were higher in cisplatin group and this difference was found to be statistically significant. The immunohistochemical staining which was done using caspase 3 and caspase 8 was revealed that immunoreactive cells were statistically higher in cisplatin group than cisplatin + edaravone group.

Conclusion

Edaravone seems to be effective in prevention of ovarian damage and short-term treatment.

Access options

Buy single article

Instant access to the full article PDF.

39,95 €

Price includes VAT (Indonesia)

References

1. Gerald KMcE, Pharm D (2006) AHFS drug information, Bethesda, Maryland

2. Kayaalp O (2005) Türkiye İlaç Kılavuzu TİK-4 2005 Formülleri. Turgut Yayıncılık, Ankara

3. Chtourou Y, Aouey B, Kebieche M, Fetoui H (2015) Protective role of naringin against cisplatin induced oxidative stress, inflammatory response and apoptosis in rat striatum via suppressing ROS-mediated NF-κB and P53 signaling pathways. Chem Biol Interact 239:76–86

   CAS  Article  Google Scholar

4. Kim JH, Jeong SJ, Kwon HY, Park SY, Lee HJ (2010) Decursin prevents cisplatin-induced apoptosis via the enhancement of antioxidant enzymes in human renal epithelial cells. Biol Pharm Bull 33(8):1279–1284

   CAS  Article  Google Scholar

5. Brouwers EE, Huitema AD, Boogerd W, Beijnen JH, Schellens JH (2009) Persistent neuropathy after treatment with cisplatin and oxaliplatin. Acta Oncol 48(6):832–841

   CAS  Article  Google Scholar

6. El Gharras H (2009) Polyphenols: food sources, properties and applications—a review. Int J Food Sci Technol 44(12):2512–2518

   Article  Google Scholar

7. Masuda T, Shimazawa M, Hara H (2017) Retinal diseases associated with oxidative stress and the effects of a free radical scavenger (Edaravone). Oxid Med Cell Longev 1–14

8. Boulikas T, Vougiouka M (2003) Cisplatin and platinum drugs at the molecular level (review). Oncol Rep 10(6):1663–1682

   CAS  PubMed  Google Scholar

9. Borovskaya TG, Goldberg VE, Fomina TI, Pakhomova AV, Kseneva SI, Poluektova ME et al (2004) Morphological and functional state of rat ovaries in early and late periods after administration of platinum cytostatics. Bull Exp Biol Med 137:331–335

   CAS  Article  Google Scholar

10. Dixit M, Yang JL, Poirier MC, Price JO, Andrews PA, Arteaga CL (1997) Abrogation of cisplatin induced programmed cell death in human breast cancer cells by epidermal growth factor antisense RNA. J Natl Cancer Inst 89(5):365–373

    CAS  Article  Google Scholar

11. Ourique GM, Saccol EM, Pês TS, Glanzner WG, Schiefelbein SH, Viviane M (2016) Protective effect of vitamin E on sperm motility and oxidative stress in valproic acid treated rats. Food Chem Toxicol 95:159–167

    CAS  Article  Google Scholar

12. Kara M, Daglioglu YK, Kuyucu Y, Tuli A, Tap O (2012) The effect of edaravone on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol 162(2):197–202

    CAS  Article  Google Scholar

Download references

Acknowledgements

None declared.

Funding

Funding was provided by Erciyes Üniversitesi (Grant number: TDK-2019-9118).

Author information

Authors and Affiliations

1. Department of Histology and Embryology, Kirsehir Ahi Evran University Medical Faculty, Bagbasi Mah, Şehit Necdet Yagiz Cad. No: 143/E, Merkez, Kirsehir, Turkey

   Ozlem Kara

2. Department of Histology and Embryology, Yozgat Bozok University Medical Faculty, Yozgat, Turkey

   Emin Kaymak

3. Department of Histology and Embryology, Erciyes University Institute of Health Sciences, Kayseri, Turkey

   Birkan Yakan

Contributions

OK: project development, data collection, and manuscript writing. BY: project development, data management, and manuscript writing. EK: data collection and analysis of data. Surgical and medical practices—OK, EK. Concept—BY. Design—BY, OK. Data collection or processing—OK, EK. Analysis or ınterpretation—OK, EK. Literature search—OK. Writing—OK.

Corresponding author

Correspondence to Ozlem Kara.

Ethics declarations

Conflict of interest

The project was supported by the Erciyes University Scientific Research Projects Unit with the project code TDK-2019-9118.

Ethical approval

The planning and execution of this study was carried out in Erciyes University Faculty of Medicine, Department of Histology-Embryology. The ethical consent was approved by Erciyes University Animal Experiments Local Ethics Committee (ERU-HADYEK) with the date of 18.01.2019 and number of 19/001.

Informed consent

No informed consent was obtained, because it was an experimental animal study.

Research involving human and animal rights

Institutional and national guidelines were used for the animals. All animals were handled in accordance with these criteria.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

About this article

Cite this article

Kara, O., Kaymak, E. & Yakan, B. Protective effect of edaravone on cisplatin-induced injury in rat ovary. Arch Gynecol Obstet 306, 1673–1678 (2022). https://doi.org/10.1007/s00404-022-06538-9

Download citation

• Received: 31 October 2021

• Accepted: 16 March 2022

• Published: 31 March 2022

• Issue Date: November 2022

• DOI : https://doi.org/10.1007/s00404-022-06538-9

Keywords

• Cisplatin
• Edaravone
• Apoptosis
• Ovarium
• Rat

robertsoninack1968.blogspot.com

Source: https://link.springer.com/article/10.1007/s00404-022-06538-9

Share this post